The Mysterious Case of Bob Bacon

Our AP Biology class recently dissected fetal pigs as a way to interactively learn about the systems of the body. After doing so, we split up into groups of five to create a CSI mystery on how one of the pigs died. We created a story line, made up interactive clues, and set up a presentation where the class went to each of the stations to find the clues and attempt to figure out who killed the pig, how, and what body system was affected.

I worked with Amanda, Bharathi, Abi, and Jen S. Our system was the immune system, and we called our dead pig Bob Bacon. We set up his death as a murder mystery, starting off with a Breaking News TV Clip explaining that scientist Bob Bacon has died, and that the police had five possible suspects – all of which were his neighbors. The class was then asked to help out with investigations by visiting each of the “homes” of these suspects and determining who could be guilty based on the timeline, Bob’s medical record, and outside information. After spending about 7 minutes at each table, we reconvened as a class and asked everyone to collaborate and guess who the murderer may have been. We then released two more Breaking News Forecasts, which led the class to conclude that one of the scientists was responsible for giving Bob Bacon HIV 10 years ago, and that it was actually Bob’s cat who killed Bob by transmitting a disease. This disease, called toxoplasmosis, was able to kill Bob because his immune system was weakened from the HIV, which had eventually turned into AIDS. Here is our intro video which set up the scene for the project!

This was definitely one of the hardest projects I have ever had to do. The fact that it was a group project, even though I love all the members of our class, made it that much more difficult. Unfortunately, I was sick during most of the time that we got to work on this project during class, so our group was forced to utilize social media in order to communicate. Even with that disadvantage, I would say that we still did a great job! I enjoyed the creativity allowed through this project. I was able to throw in a lot of special touches and puns (such as the pigs name – ex. Connor Mc. Snorts) which delighted me and (hopefully) amused everyone else. It was interesting that we had to make some sort of wild connection that was so abstract that the class would most likely not be able to guess it at first, and THEN have to actually make them guess it within the time period. I am disappointed that I was not able to see the other groups’ projects (I hate being sick!) but I can only imagine that they were fantastic!

Chromatography Lab

Chromatography is the process of physically separating substances in order to be identified and analyzed. The major factor that affects chromatography is the type and saturation of the solvent being used. The dimensions of the chromatography paper, temperature of the solution, and particle size of the solution all act as factors on the rate of chromatography as well. In our particular lab, we focused on the chromatography of pigments in leaves. The purpose of the chromatography paper was to distinctly show the separation of pigments, and the purpose of the solvent is to move the pigments up the chromatography paper through capillary action in order to separate the pigments. Before testing on leaf pigments, we used chromatography to separate the pigment colors in a black marker. The results were actually quite beautiful.

photo 1

In our lab, part of the purpose was to find the Rf value of each pigment. The Rf value stands for Relative Mobility Factor, which is referring to the movement of a substance in relation to the other protein bands. It is calculated by (Dunknown)/(Dsolvent). Dunknown stands for the distance that the solute traveled up the chromatography paper and Dsolvent stands for the distance the solvent traveled up the chromatography paper. Because each substance/pigment has a different Rf value, scientists can study the value in order to identify different types of pigments.

After testing chromatography using marker, we went on the the actual lab: using chromatography to separate the pigments in plant leaves. Our results were as following:

Our group was only able to identify two types of pigments in the green leaf: Carotene (the orange band) and Chlorophyll a (the light green band). In the purple leaf, we were able to identify carotene as well, and some other unknown pigment, which made a purple color. What we noticed is that in the purple leaf, that purple pigment masked the bands of the other pigments – however, it was evident that there were still other pigments there. Our group really enjoyed this lab because it is so aesthetically appealing. However, we found it hard to identify the different colors and to be able to tell where the rings were, because they faded a great deal. In our two green leafs, the results of the Rf value were extremely close (.88 and .89) which correlates with the fact that if the conditions for a set of chromatography are consistent, the Rf value will be constant as well. In plants, pigment is the means of which the energy of the sun is captured for photosynthesis, because pigments absorb the light energy and transfer it, which excites the electrons and thus makes way for the beginning steps of photosynthesis. My major question after doing this lab is how/why plants change color in the fall. I don’t understand how pigments are added or taken away for the start of the new season, or how one suddenly becomes dominant and masks the other.

Thanks for reading! Make sure to MicroSCOPE out the rest of my blog!

*All photos taken by our lab group*

“Affect of Different Colored Lights on Photosynthesis.” Affect of Different Colored Lights on Photosynthesis. CU Boulder, n.d. Web. 04 Oct. 2014.

Light Absorption for Photosynthesis.” Light Absorption for Photosynthesis. N.p., n.d. Web. 04 Oct. 2014.